macro prep high q resin column Search Results


macro prep high q column  (Bio-Rad)
95
Bio-Rad macro prep high q column
Macro Prep High Q Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc05878146-75-8-12?v=Bio-Rad
Average 95 stars, based on 1 article reviews
macro prep high q column - by Bioz Stars, 2026-06
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pre packed mini macro prep high q cartridges  (Bio-Rad)
92
Bio-Rad pre packed mini macro prep high q cartridges
Pre Packed Mini Macro Prep High Q Cartridges, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/us08460529-252-12-18?v=Bio-Rad
Average 92 stars, based on 1 article reviews
pre packed mini macro prep high q cartridges - by Bioz Stars, 2026-06
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deae cellulose  (Bio-Rad)
94
Bio-Rad deae cellulose
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Deae Cellulose, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc11964790-26-10-16?v=Bio-Rad
Average 94 stars, based on 1 article reviews
deae cellulose - by Bioz Stars, 2026-06
94/100 stars
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anion exchange chromatography  (Bio-Rad)
93
Bio-Rad anion exchange chromatography
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Anion Exchange Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc03177201-87-4-7?v=Bio-Rad
Average 93 stars, based on 1 article reviews
anion exchange chromatography - by Bioz Stars, 2026-06
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methyl 3h sam usingmacro prep highq resin  (Bio-Rad)
93
Bio-Rad methyl 3h sam usingmacro prep highq resin
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Methyl 3h Sam Usingmacro Prep Highq Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/10__1074_slash_jbc__m112__410290-147-6-10?v=Bio-Rad
Average 93 stars, based on 1 article reviews
methyl 3h sam usingmacro prep highq resin - by Bioz Stars, 2026-06
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anionexchange supports  (Bio-Rad)
94
Bio-Rad anionexchange supports
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Anionexchange Supports, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pm12429810-177-60-66?v=Bio-Rad
Average 94 stars, based on 1 article reviews
anionexchange supports - by Bioz Stars, 2026-06
94/100 stars
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macro prep high q support  (Bio-Rad)
98
Bio-Rad macro prep high q support
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Macro Prep High Q Support, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc00114128-171-3-7?v=Bio-Rad
Average 98 stars, based on 1 article reviews
macro prep high q support - by Bioz Stars, 2026-06
98/100 stars
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macro prep  (Bio-Rad)
93
Bio-Rad macro prep
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Macro Prep, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/10__1094_slash_asbcj___2014___0120___01-55-13-16?v=Bio-Rad
Average 93 stars, based on 1 article reviews
macro prep - by Bioz Stars, 2026-06
93/100 stars
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macro prep high q ion exchange column  (Bio-Rad)
93
Bio-Rad macro prep high q ion exchange column
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Macro Prep High Q Ion Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc03858821-283-12-21?v=Bio-Rad
Average 93 stars, based on 1 article reviews
macro prep high q ion exchange column - by Bioz Stars, 2026-06
93/100 stars
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anion exchange resin  (Bio-Rad)
94
Bio-Rad anion exchange resin
Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography <t>(DEAE</t> cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration <t>of</t> <t>contaminated</t> endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.
Anion Exchange Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macro+prep+high+q+resin+column/pmc04167783-325-20-31?v=Bio-Rad
Average 94 stars, based on 1 article reviews
anion exchange resin - by Bioz Stars, 2026-06
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Image Search Results


Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography (DEAE cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration of contaminated endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.

Journal: Immunology and Cell Biology

Article Title: A hybrid protein is a functional molecule to reduce the cytokine storm caused by excessively activated macrophages

doi: 10.1111/imcb.70000

Figure Lengend Snippet: Design of a hybrid protein (hMIKO‐1) and its purification. (a) The frames of the full‐length AA sequences of hS100A8 and hS100A9 were schematically drawn by blue and gray horizontal bars, respectively. hMIKO‐1 is a hybrid protein of hS100A8 and hS100A9. The whole frame of hMIKO‐1 was as follows: 21 AA residues from the C terminus of hS100A9 were added to the C terminus of hS100A8. (b) SDS–PAGE (15% gel) was performed to confirm the purity of hMIKO‐1 in the absence of 2‐mercaptoethanol. hMIKO‐1 was purified by affinity chromatography (Ni‐agarose; Nacalai Tesque, Kyoto, Japan), ion‐exchange chromatography (DEAE cellulose; Bio‐Rad Co., Ltd, USA) and gel filtration (Sephacryl S‐300 HR). Proteins in the gel were stained with Coomassie Brilliant Blue. Lanes M and 1 were molecular markers and purified hMIKO‐1, respectively. As shown in the kit (Toxinsensor, Endotoxin Detection System; GenScript Inc., USA), the concentration of contaminated endotoxin present in the product hMIKO‐1 was less than 0.3 EU mg −1 protein. This result indicates no significant effects in subsequent experiments (data not shown). AA, amino acid; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate.

Article Snippet: Contaminated endotoxin was deleted as much as possible using a DEAE cellulose (Macro‐Prep High Q Media, Bio‐Rad, USA) column [26 mm (D) × 80 mm (L)].

Techniques: Purification, SDS Page, Affinity Chromatography, Ion Exchange Chromatography, Filtration, Staining, Concentration Assay, Polyacrylamide Gel Electrophoresis